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KMID : 1103720150720020108
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Journal of the Korean Society of Radiology
2015 Volume.72 No. 2 p.108 ~ p.114
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The Effects of Proteoglycan and Type II Collagen on T1ρ Relaxation Time of Articular Cartilage
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Choi Won-Seok
Yoo Hye-Jin
Hong Sung-Hwan
Choi Ja-Young
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Abstract
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Purpose: To evaluate the effects of proteoglycan and type II collagen within articular cartilage on T1ρ relaxation time of articular cartilage.
Materials and Methods: This study was exempted by the institutional and animal review boards, and informed consent was not required. Twelve porcine patellae were assigned to three groups of control, trypsin-treated (proteoglycan-degraded), or collagenase-treated (collagen-degraded). The T1ρ images were obtained with a 3 tesla magnetic resonance imaging scanner with a single loop coil. Statistical differences were detected by analysis of variance to evaluate the effects of the enzyme on T1ρ relaxation time. Safranin-O was used to stain proteoglycan in the articular cartilage and immunohistochemical staining was performed for type II collagen.
Results: Mean T1ρ values of the control, trypsin-treated, and collagenase-treated groups were 37.72 ± 5.82, 57.53 ± 8.24, and 45.08 ± 5.31 msec, respectively (p < 0.001). Histology confirmed a loss of proteoglycan and type II collagen in the trypsin- and collagenase-treated groups.
Conclusion: Degradation of proteoglycans and collagen fibers in the articular cartilage increased the articular cartilage T1ρ value.
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KEYWORD
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Osteoarthritis, Proteoglycan, Type II Collagen, Cartilage, T1ρ Imaging
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